Journal: Molecular medicine reports

Article Title: CXC195 induces apoptosis and endoplastic reticulum stress in human hepatocellular carcinoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.

doi: 10.3892/mmr.2015.4479

Figure Lengend Snippet: Figure 2. Effects of CXC195 on the proliferation of HepG2 cells. (A) Cell cycle distribution was examined using flow cytometry. (B and C) Protein expres sion levels of PCNA, Ki67, CDK4, cyclin D1, p21, p27 and p53 were determined using western blot analysis. GAPDH was used to confirm equal protein loading. Data are presented as the mean ± standard deviation (n=6). *P<0.05 and **P<0.01, vs. control. PCNA, proliferating cell nuclear antigen; CDK4, cyclin‑dependent kinase 4; Con, control.

Article Snippet: The membrane was blocked with 5% skimmed milk and incubated with PBS containing Tween 20 and the following primary antibodies at 1:500 dilution: Monoclonal/polyclonal mouse/rabbit anti-human proliferating cell nuclear antigen (PCNA) (cat no. sc-25280), p21 (sc-397), p27 (sc-393380), p53 (sc-126), Bcl-2 (sc-7382), Bcl-2-associated X (Bax) (sc-7480), AIF (sc-13116), Cyt-c (sc-13561), cyclooxygenase (COX)4 (sc-292052), tubulin (sc-9104), histone (sc-10806), glucose‐regulated protein (GRP)94 (sc-11402), GRP78 (sc-1050), CCAAT-enhancer-binding protein homologous protein (CHOP) (sc-575) and GAPDH (sc-365062) (Santa Cruz Biotechnology, Dallas, TX, USA) and cyclin-dependend kinase (CDK)4 (cat no. 12790), cyclin D1 (1044), phosphorylated (p)-PERK (3179), PERK (3192), eIF2α (9722), p-eIF2α (3597), ATF4 (11815), IRE1α (3294), p-ASK (3765), ASK (37626S), p-p38 (4511), p38 (9213), ATF6 (11815S), p-PI3K (4288S), PI3K (4249S), p-AKT (4060P), AKT (#2920S), mammalian target of rapamycin (mTOR) (2983S) and p-mTOR (5536S) (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4 ̊C.

Techniques: Flow Cytometry, Western Blot, Standard Deviation, Control

Journal: Cell Cycle

Article Title: Bcl2l10 , a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytes

doi: 10.1080/15384101.2016.1243630

Figure Lengend Snippet: Bcl2l10 RNAi disturbs MTOC formation in oocytes. Changes in MTOC formation after Bcl2l10 RNAi were determined by immunofluorescence staining. MI-arrested oocytes after Bcl2l10 RNAi were collected and stained with (A) T288-Aurka (n=7; Alexa 488, green channel), and (B) Pericentrin (n=13; Alexa 488, green channel). The spindle (Alexa 594, red channel) was stained with antibody directed against α-Tubulin. Pericentrin was used as an MTOC marker. DNA (blue channel) was counterstained with DAPI. Bar=10 µm. (lane 5) Higher-magnification photographs of the corresponding image are shown. Bar=5 µm.

Article Snippet: The oocytes were blocked with 3% BSA-PVA-PBS for 1 h and then incubated in 1% BSA-PVA-PBS containing a goat polyclonal anti-Bcl2l10 antibody (Santa Cruz Biotechnology; 1:20), a rabbit polyclonal anti-T288-Aurka antibody (Cell Signaling; 1:50), a rabbit polyclonal anti-Pericentrin antibody (Covance, Richmond, CA, USA; 1:50) and a mouse monoclonal anti-α-Tubulin antibody (Santa Cruz Biotechnology; 1:100) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Marker

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of white blood cells in the peripheral blood (x10 9 /l) and the ratio of CD34 + cells among the mononuclear cells (%).

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of cluster of differentiation 34+ cells in four groups of rat renal tissues (arrows indicate immunohistochemical staining; magnification, x200). (A–C) The (A) normal control, (B) treatment control and (C) model groups at day 5; (D–F) the model group at (D) day 10, (E) day 17 and (F) day 24; (G–J) the treatment group at (G) day 5, (H) day 10, (I) day 17 and (J) day 24.

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques: Immunohistochemical staining, Staining